Oligomerization of Recombinant Penton Base of Adenovirus Type 2 and Its Assembly with Fiber in Baculovirus-Infected Cells
Identifieur interne : 000353 ( France/Analysis ); précédent : 000352; suivant : 000354Oligomerization of Recombinant Penton Base of Adenovirus Type 2 and Its Assembly with Fiber in Baculovirus-Infected Cells
Auteurs : Lucie Karayan [France] ; Bernard Gay [France] ; Jacqueline Gerfaux [France] ; Pierre A. Boulanger [France]Source :
- Virology [ 0042-6822 ] ; 1994.
Abstract
Abstract: Full-length Ad2 penton base (PbFL571) and amino-terminal (PbAT69) and carboxy-terminal deletion mutants (PbCT550 and PbCT531) were expressed at high levels in recombinant baculovirus-infected insect cells. PbFL571, and with a lesser efficiency PbAT69, assembled penton base capsomers (9S pentamers), whereas pentamer assembly was abolished with the deletion of the 21 C-terminal amino acids (as in PbCT550). A discrete population of penton base capsomers in PbFL571 and PbAT69 was found to occur as 12S decamers. PbFL571 and PbAT69 penton base were able to bind to full-length (but not to N-terminal deletion mutants of) fiber trimer to form authentic penton capsomer when coexpressed in trans in double-infected cells. Penton base monomers accumulated by PbCT550 and PbCT531 were capable of interacting with both fiber trimers and monomers. This suggested that mutual binding sites exist in the fiber and penton base subunits, and that the fiber-binding domain is located between residues 70 and 531 in the penton base, with its complementary domain situated at the N-terminus of the fiber. Intranuclear inclusions of recombinant protein were observed with the three deletion mutants PbAT69, PbCT550, and PbCT531, implying the existence of karyophilic signal(s) in the central domain of penton base.
Url:
DOI: 10.1006/viro.1994.1400
Affiliations:
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Boulanger</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:14549E3ADBFA9B8B8122B30B17D8993C1B55792F</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1006/viro.1994.1400</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-LF7Q1R6G-R/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000826</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000826</idno><idno type="wicri:Area/Istex/Curation">000826</idno><idno type="wicri:Area/Istex/Checkpoint">001A52</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001A52</idno><idno type="wicri:doubleKey">0042-6822:1994:Karayan L:oligomerization:of:recombinant</idno><idno type="wicri:Area/Main/Merge">004478</idno><idno type="wicri:Area/Main/Curation">004415</idno><idno type="wicri:Area/Main/Exploration">004415</idno><idno type="wicri:Area/France/Extraction">000353</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Oligomerization of Recombinant Penton Base of Adenovirus Type 2 and Its Assembly with Fiber in Baculovirus-Infected Cells</title><author><name sortKey="Karayan, Lucie" sort="Karayan, Lucie" uniqKey="Karayan L" first="Lucie" last="Karayan">Lucie Karayan</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Gay, Bernard" sort="Gay, Bernard" uniqKey="Gay B" first="Bernard" last="Gay">Bernard Gay</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Gerfaux, Jacqueline" sort="Gerfaux, Jacqueline" uniqKey="Gerfaux J" first="Jacqueline" last="Gerfaux">Jacqueline Gerfaux</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Boulanger, Pierre A" sort="Boulanger, Pierre A" uniqKey="Boulanger P" first="Pierre A." last="Boulanger">Pierre A. Boulanger</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Virologie et Pathogénèse Moléculaires (CNRS URA 1487), Faculté de Médecine, Boulevard Henri IV, 34060 Montpellier</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">202</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="782">782</biblScope><biblScope unit="page" to="795">795</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Full-length Ad2 penton base (PbFL571) and amino-terminal (PbAT69) and carboxy-terminal deletion mutants (PbCT550 and PbCT531) were expressed at high levels in recombinant baculovirus-infected insect cells. PbFL571, and with a lesser efficiency PbAT69, assembled penton base capsomers (9S pentamers), whereas pentamer assembly was abolished with the deletion of the 21 C-terminal amino acids (as in PbCT550). A discrete population of penton base capsomers in PbFL571 and PbAT69 was found to occur as 12S decamers. PbFL571 and PbAT69 penton base were able to bind to full-length (but not to N-terminal deletion mutants of) fiber trimer to form authentic penton capsomer when coexpressed in trans in double-infected cells. Penton base monomers accumulated by PbCT550 and PbCT531 were capable of interacting with both fiber trimers and monomers. This suggested that mutual binding sites exist in the fiber and penton base subunits, and that the fiber-binding domain is located between residues 70 and 531 in the penton base, with its complementary domain situated at the N-terminus of the fiber. Intranuclear inclusions of recombinant protein were observed with the three deletion mutants PbAT69, PbCT550, and PbCT531, implying the existence of karyophilic signal(s) in the central domain of penton base.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region><settlement><li>Montpellier</li></settlement></list><tree><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Karayan, Lucie" sort="Karayan, Lucie" uniqKey="Karayan L" first="Lucie" last="Karayan">Lucie Karayan</name></region><name sortKey="Boulanger, Pierre A" sort="Boulanger, Pierre A" uniqKey="Boulanger P" first="Pierre A." last="Boulanger">Pierre A. Boulanger</name><name sortKey="Gay, Bernard" sort="Gay, Bernard" uniqKey="Gay B" first="Bernard" last="Gay">Bernard Gay</name><name sortKey="Gerfaux, Jacqueline" sort="Gerfaux, Jacqueline" uniqKey="Gerfaux J" first="Jacqueline" last="Gerfaux">Jacqueline Gerfaux</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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